Protein Purification Reagents and Kits
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
Thermo Scientific™ Pierce™ Protein Refolding Kit
Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.
Thermo Scientific™ AminoLink™ Plus Micro Immobilization Kit, 0.1 mL
Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.
Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit
Easily capture and affinity purify binding partners of His-tagged fusion proteins using nickel-chelate (Ni-IDA) agarose beads and this complete reagent set.
Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit
Easily capture and affinity-purify binding partners of any biotin-labeled protein or other ligand using these streptavidin agarose beads and complete reagent set.
Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit
Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.
Thermo Scientific™ Pierce™ Direct IP Kit
Immobilize IP antibodies (any species or class) on agarose beads without Protein A/G to immunoprecipitate antigens without antibody interference.
Thermo Scientific™ Inclusion Body Solubilization Reagent
Retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with this reagent optimized for inclusion body solubilization.
Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit
Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.
Thermo Scientific™ Pierce™ Control Agarose Resin
Unmodified beaded agarose resin for pre-clearing protein immunoprecipitation samples to reduce background signal and nonspecific binding in IP assays.
Cytiva PD MidiTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Thermo Scientific™ Pierce™ GST Spin Purification Kit, 1 mL
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues
Fractionate tissue sample-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins within 2 hours.